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目的了解阿托品对氧乐果中毒小鼠全血胆碱酯酶及脑胆碱酯酶急性重活化的作用。方法昆明种小鼠30只,雄雌各半,随机分成对照组(生理盐水)、氧乐果+生理盐水组、氧乐果+阿托品组3组,每组10只,皮下注射,20min后处死动物,采集样品,采用三氯化铁分光光度法测定全血和脑胆碱酯酶活力。结果 3组全血胆碱酯酶活力百分数方差分析结果显示,差异有统计学意义(F=5.854,P<0.01);两两比较显示,对照组(生理盐水)和氧乐果+生理盐水组全血胆碱酯酶活力百分数差异有统计学意义(P<0.01)。3组脑胆碱酯酶活力百分数方差分析结果显示,差异有统计学意义(F=3.667,P<0.05);两两比较显示,对照组(生理盐水)和氧乐果+生理盐水组脑胆碱酯酶活力百分数差异有统计学意义(P<0.05)。结论氧乐果中毒对小鼠全血和脑胆碱酯酶活力产生抑制,阿托品对氧乐果中毒小鼠全血和脑胆碱酯酶有重活化作用。
Objective To investigate the effect of atropine on whole blood cholinesterase and acute reactivation of brain cholinesterase in mice with omethoate poisoning. Methods Thirty Kunming mice were randomly divided into 3 groups: control group (normal saline), omethoate + normal saline group, omethoate + atropine group, 10 in each group, injected subcutaneously and sacrificed 20 minutes later Animals were collected samples, the determination of whole blood and brain cholinesterase activity using ferric chloride spectrophotometry. Results The variance analysis of percentages of cholinesterase in the three groups showed that the difference was statistically significant (F = 5.854, P <0.01). The comparison between two groups showed that in the control group (normal saline) and omethoate + saline group The percentage of cholinesterase in whole blood had statistical significance (P <0.01). The results of variance analysis of percentages of brain cholinesterase in group 3 showed that the difference was statistically significant (F = 3.667, P <0.05). The comparison between two groups showed that in the control group (normal saline) and the omethoate + The percentage of alkaline esterase activity had significant difference (P <0.05). Conclusion Omethoate inhibits the whole blood and brain cholinesterase activity in mice, and atropine has a reactivation effect on whole blood and brain cholinesterase in omethoate-induced mice.