目的 :观察 rh TNF- α在体外对正常人精子的运动及其超微结构的影响。方法 :2 6例健康成年男性手淫法获得精液。Percoll梯度离心法得到的精子为精子模型 ,将精子密度调整为 1 0× 1 0 6 /ml;在 37℃、5 % CO2 的环境中 ,分别与终浓度为 60 pg/ml、90 pg/ml、2 70 pg/ml的 rh TNF- α孵育 ,同时设立对照组。在 0 .5 h和 4h时间段取样 ,CASA观察实验组和对照组精子的运动能力 ,TEM观察实验组和对照组精子的超微结构。结果 :与对照组相比较 60 pg/ml、90 pg/ml、2 70 pg/ml各组的精子运动能力明显降低 ( P<0 .0 1 )。精子超微结构发生程度不同的变化 :细胞膜变薄、扭曲或呈波浪状改变 ,大部分精子细胞膜不连续 ;细胞膜与顶体外膜间隙增宽 ,透亮度增强 ,顶体变厚 ,甚至可见顶体外膜破裂 ,顶体内容物流失 ;线粒体密度降低 ,透光度增强 ,严重者线粒体外形变圆 ,内有空泡形成 ;核膜与核内染色质未发生改变 ;纤维轴丝 9+ 2模式未改变。结论 :rh TNF- α可降低精子运动能力 ,同时破坏精子的顶体和膜结构而降低精子受精能力 Objective: To observe the effects of rh TNF-α on the motility and ultrastructure of normal human sperms in vitro. Methods: 26 healthy adult male masturbation obtained semen. Percoll gradient centrifugation of sperm as a sperm model, the sperm density adjusted to 1 0 × 106 / ml; 37 ℃, 5% CO2 environment, respectively, with a final concentration of 60 pg / ml, 90 pg / ml , 2 70 pg / ml of rh TNF-α incubation, while the establishment of the control group. Samples were taken at 0.5 h and 4 h time points. CASA was used to observe the motility of sperm in experimental and control groups. The ultrastructure of sperm in experimental group and control group was observed by TEM. Results: Compared with the control group, the sperm motility of 60 pg / ml, 90 pg / ml and 2 70 pg / ml groups decreased significantly (P <0.01). Sperm ultrastructural changes occur in different degrees: the membrane thinning, distorted or wavy changes, most of the sperm cell membrane is not continuous; cell membrane and the perpetural membrane gap widens, enhance the brightness, acrosome thickened, or even see the acrosome Membrane rupture, loss of acrosome content; mitochondrial density decreased, transmittance increased, severe mitochondrial round shape, the formation of vacuoles; nuclear membrane and nuclear chromatin did not change; fiber axis wire 9 + 2 mode change. Conclusion: rh TNF-α can reduce sperm motility, at the same time destroy sperm acrosome and membrane structure and reduce sperm fertility