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目的:建立测定5-氟尿嘧啶核苷(5-FUR)前药脂质体中磷脂含量的方法。方法:以乙醇裂解5-FUR前药脂质体,用氯仿萃取后,在488nm波长处用硫氰铁铵显色法测定脂质体中磷脂的含量。结果:磷脂检测浓度的线性范围为0.01~0.10mg·mL-1(r=0.9996,n=3);平均回收率为99.66%(RSD=3.17%)。结论:本方法简便、快速、灵敏,可用于5-FUR前药脂质体的质量控制。
Objective: To establish a method for the determination of phospholipids in 5-fluorouridine (5-FUR) prodrug liposomes. Methods: The liposomes of 5-FUR prodrug were lysed by ethanol and extracted with chloroform. The content of phospholipid in liposomes was determined by thiocyanate colorimetry at 488nm wavelength. Results: The linear range of phospholipid detection was 0.01-0.10 mg · mL -1 (r = 0.9996, n = 3). The average recovery was 99.66% (RSD = 3.17%). Conclusion: The method is simple, rapid and sensitive and can be used for quality control of 5-FUR prodrug liposomes.