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目的:研究基质金属蛋白酶-12(MMP-12)蛋白及其基因片段在喉鳞状细胞癌中的表达情况及其与喉癌生物学行为的关系。方法:选取30例声门上喉癌新鲜组织标本(实验组)及0.5cm外正常黏膜组织(对照组),按标本恶性度低、中、高度不同,分别制作不同恶性度的基因芯片,研究其上游基因NM-002426表达比率;用免疫组织化学的方法检测其在组织中的表达情况。结果:NM-002426基因在3种不同恶性度基因芯片中都为高表达比率,较同族的基因片段比率均高,在中、高度恶性芯片中的比率是相应芯片中测定结果中的最高值;免疫组织化学结果显示实验组与对照组MMP-12表达率差异有统计学意义(P<0.01),实验组中转移者与未转移者表达差异有统计学意义(P<0.05);而不同恶性度、不同性别间差异无统计学意义。结论:在喉鳞状细胞癌的发生、发展中,NM-002426基因显著增殖,编码产生的大量MMP-12对肿瘤的转移起到了关键作用;MMP-12及其基因的高表达可作为喉癌的辅助诊断依据。
Objective: To investigate the expression of matrix metalloproteinase-12 (MMP-12) and its gene fragments in laryngeal squamous cell carcinoma (LSCC) and its relationship with the biological behavior of laryngeal carcinoma. Methods: Thirty fresh specimens of laryngeal carcinoma of the supraglottic (experimental group) and normal mucosa of 0.5 cm (control group) were selected. Gene microarrays with different malignant degrees were made according to the low, medium and high degree of malignancy. Its upstream gene NM-002426 expression ratio; using immunohistochemistry to detect the expression in the tissue. Results: The high expression rate of NM-002426 gene in all 3 different malignant gene chips was higher than that of the same gene fragments. The ratio of NM-002426 in the medium and high-density chip was the highest in the corresponding chip. The results of immunohistochemistry showed that the expression of MMP-12 in the experimental group and the control group was significantly different (P <0.01), but there was significant difference between the metastasis group and the non-metastasis group (P <0.05) Degree, gender differences were not statistically significant. CONCLUSIONS: The NM-002426 gene is significantly proliferated in the development and progression of laryngeal squamous cell carcinoma, and a large number of MMP-12 encoded plays a key role in tumor metastasis. The high expression of MMP-12 and its gene can be used as a marker of laryngeal cancer The diagnosis of the basis.