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为研究１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙氨基）丙烷盐酸盐（ＤＤＰＨ）对心肌肥厚大鼠左室组织ＤＮＡ含量有无逆转作用，用部分狭窄腹主动脉的方法建立心肌肥厚的模型．术后ｗｋ４开始给药，连续８ｗｋ，左室心肌切片并进行Ｆｅｕｌｇｅｎ染色，用ＴＪＴＹ－３００图像分析系统对ＤＮＡ进行相对定量，发现肥厚组平均吸光度为０．０８９，是对照组的１．４３倍，而二个给药组分别为０．０７９和０．０７１，明显低于肥厚组，但仍高于对照组（Ｐ＜０．０５），说明ＤＤＰＨ对肥厚心肌的ＤＮＡ含量有一定的逆转作用．为进一步研究ＤＤＰＨ能否抑制去甲肾上腺素（ＮＥ）致培养乳鼠心肌细胞ＤＮＡ合成的作用，体外培养乳鼠心肌细胞，实验分６组：（１）对照组；（２）ＮＥ组；（３）ＤＤＰＨ１．０μｍｏｌ·Ｌ－１组；（４）ＤＤＰＨ１０μｍｏｌ·Ｌ－１组；（５）哌唑嗪（Ｐｒａ）１．０μｍｏｌ·Ｌ－１组；６）Ｐｒａ１０μｍｏｌ·Ｌ－１组．每孔加［３Ｈ］ＴｄＲ３７Ｂｑ，３ｈ后测ｃｐｍ值，发现ＮＥ组为对照组的３．１倍，而ＤＤＰＨ及Ｐｒａ组均减少ｃｐｍ值（Ｐ＜０．０１），且Ｐｒａ组作用更明显，二个高剂量组与对照组无显著差异，结果说明ＤＤＰＨ可以抑制ＮＥ诱导?
To investigate the effects of 1- (2,6-dimethylphenoxy) -2- (3,4-dimethoxyphenethylamino) propane hydrochloride (DDPH) on left ventricular tissue DNA content in hypertrophic rats No reversal effect, using part of the narrow abdominal aorta method to establish a model of cardiac hypertrophy. After wk4 began to be administered, continuous 8wk, left ventricular myocardial slices and Feulgen staining, TJTY-300 image analysis system for DNA relative quantification and found that the average absorbance of hypertrophy group was 0.089, 1.43 times that of the control group , While the two administration groups were 0.079 and 0.071, respectively, which were significantly lower than those in hypertrophied group (P <0.05), indicating that DDPH reversed the DNA content of hypertrophic myocardium . In order to further study whether DDPH can inhibit the DNA synthesis of cultured neonatal rat cardiomyocytes induced by norepinephrine (NE), neonatal rat cardiomyocytes were cultured in vitro. The experiment was divided into 6 groups: (1) control group; (2) NE group; ( 3) DDPH1.0μmol·L-1 group, (4) DDPH10μmol·L-1 group, (5) prazosin (Pra) 1.0μmol·L-1 group, and 6) Pra10μmol·L-1 group. The [cetyltrimethylammonium chloride (3H) TdR37Bq was added to each well, and the cpm value was measured after 3h. It was found that the NE group was 3.1 times as much as the control group, while the cpm values were decreased in DDPH and Pra groups (P <0.01) The two high-dose group and the control group no significant difference, the results show that DDPH can inhibit NE induced?