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目的:优选鳖甲活性多肽的提取工艺及膜分离工艺。方法:以多肽提取量和浸膏得率为指标,通过正交试验考察料液比、提取时间、提取次数对鳖甲多肽提取工艺的影响;以膜渗透通量或膜效能为指标,运用单因素试验优选鳖甲活性多肽的膜分离工艺。结果:最佳提取工艺为醋鳖甲粉碎过24目筛,加6倍量水提取3次,每次3 h;多肽提取量达2.442 g,浸膏得率11.71%。膜分离纯化条件为鳖甲多肽质量分数6%,截留相对分子质量20,8 kD的超滤膜操作压力分别为0.18~0.21,0.8 MPa;蛋白质截留率83.67%,相对分子质量<8 000的鳖甲多肽纯度达57.62%。结论:膜分离技术可用于鳖甲活性多肽的制备。
Objective: To optimize the extraction technology and membrane separation technology of the active peptides from turtle shell. Methods: The extraction rate of polysaccharides and the yield of extract were used as indexes. The effects of the ratio of material to liquid, the extraction time and the number of extraction on the extraction process of Biejia peptide were investigated by orthogonal test. Using membrane permeation flux or membrane efficiency as index, Optimization of Factor Tests for Separation of Bioactive Peptides from Turtle Acipenser. Results: The optimum extraction process was vinegar, turtle shell crushed through 24 mesh sieve, 6 times the amount of water extracted three times, each time 3 h; peptide extraction amounted to 2.442 g, extract rate of 11.71%. Membrane separation and purification conditions for the turtle shell peptide mass fraction of 6%, the relative molecular mass cut off 20,8 kD ultrafiltration membrane operating pressure was 0.18 ~ 0.21,0.8 MPa; protein retention rate of 83.67%, relative molecular mass <8 000 of turtle A polypeptide purity of 57.62%. Conclusion: Membrane separation technology can be used for the preparation of active peptides from turtle.