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目的:建立解郁和肝胶囊中栀子苷和芍药苷的HPLC含量测定方法。方法:用Diamonsil-C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.1%磷酸溶液(14∶86)为流动相;检测波长为238 nm;柱温为25℃;流速为1.0 mL·min-1。结果:栀子苷进样量在0.16～2.42μg范围内,进样量与峰面积呈良好的线性关系,r=0.999 7,平均回收率为99.25%(n=6);芍药苷进样量在0.12～1.82μg范围内,进样量与峰面积呈良好的线性关系,r=0.999 5,平均回收率为98.87%(n=6)。结论:本方法操作简便,结果准确,重现性好,可作为该制剂的含量测定方法。
Objective: To establish HPLC method for determination of geniposide and paeoniflorin in Jieyu Hegan capsules. METHODS: Diamonsil-C18 column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-0.1% phosphoric acid solution (14:86) as the mobile phase. The detection wavelength was 238 nm. The column temperature was 25 ℃ and the flow rate was 1.0 mL · Min-1. Results: The injection of geniposide was in the range of 0.16-2.42μg with a good linear relationship between injection volume and peak area (r = 0.999 7, the average recovery was 99.25% (n = 6)). The concentration of paeoniflorin In the range of 0.12-1.82μg, the injection volume and the peak area showed a good linear relationship, r = 0.999 5, the average recovery was 98.87% (n = 6). Conclusion: The method is simple, accurate, reproducible and can be used as a method for determination of the content of the preparation.