目的了解郴州市2006～2008年流感流行状况及流感毒株的型别分布,分析其流行趋势,为流感防制提供科学依据。方法采集流感样病例(ILI)的咽拭子标本,用传代狗肾细胞(MDCK)进行病毒分离,采用血凝抑制实验(HI)进行流感病毒型别鉴定。结果3年全市4家哨点医院三年共采3 124份ILI咽拭子标本,分离到毒株383株,分离阳性率为10.82%,经国家流感中心最后复核鉴定的结果为:H1N1亚型76株,H3N2亚型181株,B型105株,未分型21株;流感及ILI暴发疫情病例咽拭子标本78份,分离到流感病毒39株,分离阳性率为50.00%,分型鉴定结果为:H1N1亚型2株,H3N2亚型5株,B型32株。结论郴州市流感监测地区2006-2008年均有流感活动,2006年,HIN1亚型为优势株,2007年H3N2亚型为优势株,2008年,HIN1亚型和H3N2亚型为优势株。而暴发疫情则主要由B型流感病毒引起。 Objective To understand the prevalence of influenza and the type distribution of influenza strains in Chenzhou from 2006 to 2008, analyze the epidemic trend and provide a scientific basis for the prevention and control of influenza. Methods Throat swab samples of influenza-like illness (ILI) were collected, and virus was isolated by passaged dog kidney cells (MDCK). Influenza type was identified by hemagglutination inhibition test (HI). Results In 3 years, 4 124 sentinel swabs were collected from 4 sentinel hospitals in the city. 383 strains of ILI were isolated and the positive rate was 10.82%. The results of the final review by the National Influenza Center were: H1N1 subtype 76 strains, 181 strains of H3N2 subtype, 105 strains of B type and 21 strains of non-subtype type. Throat swab samples of influenza and ILI outbreak were 78, and 39 strains of influenza virus were isolated. The positive rate was 50.00% The result was: 2 H1N1 subtypes, 5 H3N2 subtypes and 32 B type. Conclusion Influenza activity was found in all the influenza surveillance areas in Chenzhou from 2006 to 2008. In 2006, HIN1 subtype was the predominant strain. In 2007, H3N2 subtype was dominant strain. In 2008, HIN1 subtype and H3N2 subtype were predominant strains. The outbreak is mainly caused by influenza B virus.