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目的筛选适合分离和纯化沙枣花总黄酮的大孔吸附树脂并确立纯化工艺参数。方法以大孔吸附树脂吸附率及解吸率为指标,筛选出富集沙枣花总黄酮的最佳树脂,并通过单因素考察该树脂分离、纯化沙枣花总黄酮的最佳工艺条件。结果 DA201树脂对沙枣花总黄酮有良好的吸附分离性能。其具体工艺条件为:树脂柱径高比为1∶8、控制上样流速为0.5mL.min-1、上样液浓度20 mg.mL-1、调pH为4、洗脱流速为0.5 mL.min-1、最大上样为4.5 BV、洗脱剂浓度80%、洗脱剂用量12 BV。结论 DA201型树脂在所确定的工艺条件下,纯化沙枣花总黄酮效果良好,总黄酮得率达到85.23%,总黄酮纯度达到了14.95%,比粗提样品纯度(4.901%)提高了3倍。
Objective To screen the macroporous resin suitable for the separation and purification of total flavonoids of Elaeagnus angustifolia and to establish the purification process parameters. Methods Based on the adsorption and desorption rate of macroporous adsorption resin, the best resin for enrichment of the total flavonoids of C. armeniacum was screened out. The optimum conditions for the isolation and purification of the total flavonoids from E. olgensis were investigated by single factor. Results DA201 resin has good adsorption and separation properties on the total flavonoids of Elaeagnus angustifolia. The specific process conditions were as follows: the resin column diameter to height ratio was 1: 8, the flow rate of control sample was 0.5mL.min-1, the concentration of sample solution was 20 mg.mL-1, the pH was adjusted to 4 and the elution flow rate was 0.5 mL . min-1, the maximum sample was 4.5 BV, the eluent concentration was 80% and the eluent dosage was 12 BV. Conclusion The results showed that the total flavonoids yield of DA201 resin was 85.23%, the purity of total flavonoids reached 14.95%, which was 3 times higher than that of the crude extract (4.901%) under the determined process conditions. .