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研究不同浓度的维生素 E(VE)和β-胡萝卜素 (βC)对 Cu2 + 诱导的氧化修饰低密度脂蛋白 (L DL )作用的影响 ,通过测定硫代巴比妥酸反应物质 (TBARS)、L DL 的电泳迁移率 (Rf)以及荧光物质 (L ipofusin)扫描 ,反映 L DL的氧化修饰程度。结果表明 :VE、βC均可减少 TBARS的产生、减小 L DL的 Rf,并且具有剂量 -效应关系 ,随浓度增加 ,VE抑制作用加强而 βC抑制作用减弱 ,而且 VE对 TBARS的影响比 βC小 ,对 Rf的影响比βC大。二者对荧光物质均有降低作用 ,但无剂量 -效应关系。提示 VE、βC均可不同程度的抑制Cu2 +氧化修饰 L DL 的作用 ,在降低机体脂质过氧化反应、减少氧化修饰型 L DL(OX- L DL)的形成方面具有重要作用 ,但二者作用机理可能并不相同
The effects of different concentrations of vitamin E (VE) and β-carotene (βC) on Cu2 + -induced oxidatively modified low density lipoprotein (LDL) were studied. The effects of thiobarbituric acid reactive substances (TBARS) The electrophoretic mobility (Rf) of L DL and L ipofusin scans reflect the degree of oxidative modification of L DL. The results showed that both VE and βC could reduce the production of TBARS, decrease the Rf of L DL, and have a dose-effect relationship. With the increase of concentration, the inhibition of VE was enhanced and the inhibition of βC was weakened, and the effect of VE on TBARS was smaller than that of βC , The impact of Rf than βC large. Both of the fluorescent substances have reduced the role, but no dose-effect relationship. These results suggest that both VE and βC may inhibit Cu2 + -oxidative LDL to some extent and play an important role in reducing body lipid peroxidation and reducing the formation of oxidized modified L DL (OX-L DL) The mechanism of action may not be the same