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构建了基于Activator/Dissociation(β-glucuronidase)[简称Ac/Ds(GUS)]结构的捕获质粒p13B,用于分离水稻基因启动子。以此质粒用农杆菌介导的方法转化粳稻品种中花11的胚性愈伤组织,对获得的18个独立转化株的T2代植株进行了抗除草剂筛选,从141个抗除草剂转基因植株中用PCR方法检测到其中37株是Ds因子发生了转座的植株,而且这种转座到新位置上的Ds因子是遗传的。初步观察到其中5株的GUS染色呈阳性。
A capture plasmid p13B based on the Activator / Dissociation (Ac-DsgS) structure was constructed for the isolation of rice gene promoter. The calli were transformed into embryogenic callus of Zhonghua 11 by Agrobacterium tumefaciens method, and the herbicide resistant plants of T2 generation of 18 independent transformants were screened. From 141 herbicide-resistant transgenic plants Among them, 37 plants were transposed with Ds factor by PCR, and the Ds factor transposed to the new position was inherited. Preliminary observation showed that 5 of them were positive for GUS staining.