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目的:分析儿童急性髓系白血病(AML)免疫表型特征,探讨其临床意义。方法:采用四色流式细胞术CD45/SSC双参数散点图设门方法,对127例儿童AML患者幼稚细胞进行免疫表型检测,对抗原表达情况进行分析。结果:在127例儿童急性髓系白血病患者中,髓系特异性抗原CD33、CD13和CD117的表达最常见,分别达95.3%、90.6%、90.6%。造血干/祖细胞抗原CD34、HLA-DR、CD38阳性率分别达53.5%、71.6%和97.6%。有65.4%的病例伴有淋系抗原的表达,其中以CD56的表达最常见占38.6%,其次为CD7占21.3%。有淋系抗原表达(LymAg~+)患者早期抗原CD34和HLA-DR抗原表达阳性率明显高于无淋系抗原表达(LymAg~-)患者(P<0.05)。结论:免疫分型对儿童AML的诊断和不同亚型鉴别具有重要意义。
Objective: To analyze the immunophenotypic characteristics of children with acute myeloid leukemia (AML) and to investigate its clinical significance. Methods: Four-color flow cytometry CD45 / SSC double-parameter scatter plot method was used to detect the immature cells in 127 children with AML. The antigen expression was analyzed. Results: The expression of myeloid specific antigens CD33, CD13 and CD117 were the most common in 127 children with acute myeloid leukemia, accounting for 95.3%, 90.6% and 90.6% respectively. The positive rates of hematopoietic stem / progenitor antigen CD34, HLA-DR and CD38 were 53.5%, 71.6% and 97.6% respectively. There were 65.4% of cases with lymphoid antigen expression, of which the expression of CD56 most commonly accounted for 38.6%, followed by CD7 accounted for 21.3%. The positive rate of early antigen CD34 and HLA-DR antigen expression in LymAg ~ + patients was significantly higher than that in LymAg ~ (+) patients (P <0.05). Conclusion: Immunophenotyping is of great importance for the diagnosis of AML in children and identification of different subtypes.