目的　克隆血管生成抑制因子Arresten基因 ,测定并分析其基因序列。方法　从人胎盘组织中提取总RNA ,经逆转录 聚合酶链式反应 (RT PCR)扩增出Arresten基因 ;采用T A突出端克隆 ,构建克隆载体 pGEM Arr ,测定Arresten基因序列。 结果　Arresten基因的克隆中 ,以特异引物Pr2为反转录引物的Arresten基因的扩增比用OligodT的效果好。其序列测定证实Arresten基因克隆成功。结论　Arresten基因需用适当方法克隆 ,是抗血管生成及肿瘤生成机制研究的基础 Objective To clone the angiogenesis inhibitor Arresten gene and determine its gene sequence. Methods Total RNA was extracted from human placenta tissue and Arresten gene was amplified by reverse transcription polymerase chain reaction (RT PCR). Clone vector pGEM Arr was constructed by using T A overhang, and the Arresten gene sequence was determined. Results Arresten gene cloning, with specific primer Pr2 as a reverse transcription primer Arresten gene amplification than OligodT effect. The sequencing confirmed that the Arresten gene cloned successfully. Conclusion The Arresten gene needs to be cloned by appropriate methods and is the basis for the study of anti-angiogenesis and tumorigenesis