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在pH =6 86磷酸盐缓冲溶液中 ,采用电化学 (循环伏安法、微分脉冲伏安法和交流阻抗及数据拟合技术 )、粘度测定、电子吸收光谱和溴化乙锭 (EB)荧光分析法研究了 [Cu(phen) (H2 O) (L Met) ] + (phen =1,10 邻菲咯啉 ,L Met =L 蛋氨酸 )与小牛胸腺DNA的相互作用 .结果发现中心铜离子在循环伏安图上呈现 1对明显的准可逆氧化还原波 .当加入一定量的DNA时 ,配合物的氧化还原峰电流明显降低 ,扩散系数减小 ,电化学反应电阻增大 ,电子光谱的最大吸收峰明显红移 ,产生明显的减色效应 ,同时 ,配合物也能较大程度地猝灭EB DNA体系的荧光 ,说明 [Cu(phen) (H2 O) (L Met) ] + 与DNA的作用较强 ,作用模式为部分插入作用
Electrochemical (cyclic voltammetry, differential pulse voltammetry and AC impedance and data fitting techniques), viscosity measurement, electron absorption spectroscopy and ethidium bromide (EB) fluorescence were used in a pH = 6 86 phosphate buffer solution The interaction between [Cu (phen) (H2 O) (L Met)] + (phen = 1,10-phenanthroline and L Met = L methionine) and calf thymus DNA was investigated by analytical method. A significant quasi-reversible oxidation-reduction wave appeared on the cyclic voltammogram. When a certain amount of DNA was added, the redox peak current of the complex decreased obviously, the diffusion coefficient decreased, the electrochemical reaction resistance increased, and the electron spectral The maximum absorption peak shifts red-shifted significantly, resulting in obvious color reduction effect. At the same time, the complex also quenches the fluorescence of EB DNA system to a great extent, indicating that [Cu (phen) (H2 O) (L Met) The role of a strong role model as part of the role of insertion