目的建立固相萃取-高效液相色谱法测定氯雷他定血药浓度的方法。方法采用ODS-C18小柱处理样品,Zorbax SB-C18硅烷键合硅胶柱(4.6mm×250mm,5um),流动相:50mmol.L-1磷酸二氢铵缓冲液(磷酸调PH4.0)-乙腈(62∶38),流速:1.2mL.m in-1,检测波长:275nm。结果线性范围1~100 ng.mL-1(r=0.999 6),回收率在82.63%~92.41%,日内、日间RSD<10%,最低定量浓度为1ng.mL-1。结论该法简便、快速、准确,适用于氯雷他定药代动力血和生物等效性研究。 Objective To establish a method for the determination of loratadine concentration by high performance liquid chromatography. Methods The sample was treated with ODS-C18 cartridge, and the mobile phase was Zorbax SB-C18 silane-bonded silica column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of 50 mmol·L -1 ammonium dihydrogen phosphate buffer Acetonitrile (62:38), flow rate: 1.2 mL.m in-1, detection wavelength: 275 nm. Results The linear range was 1 ~ 100 ng.mL-1 (r = 0.999 6). The recoveries ranged from 82.63% to 92.41%. The intra-day and inter-day RSD was less than 10% and the lowest concentration was 1 ng.mL-1. Conclusion The method is simple, rapid and accurate and is suitable for the study of loratadine pharmacokinetics and bioequivalence.