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目的:观察甲状腺癌(TC)细胞中微小RNA-630(miR-630)对上皮-间充质转化(EMT)标志物锌指转录因子(Slug)表达的影响，及对细胞侵袭能力的影响。方法:收集河北医科大学第二医院普外科2018年1月至2019年12月之间的40例乳头状甲状腺癌及其癌旁标本。采用荧光定量聚合酶链反应(PCR)的方法检测甲状腺癌及其癌旁组织中miR-630的表达水平；将miR-630模拟物(mimics)转染至人乳头瘤状甲状腺癌细胞(TPC-1)细胞中，应用荧光定量PCR的方法观察Slug基因的表达水平，使用双荧光素酶报告基因实验观察miR-630对Slug基因的调控作用；同时，将过表达Slug质粒与miR-630 mimics共转染至TPC-1细胞，应用Transwell细胞侵袭实验观察miR-630和Slug表达变化对甲状腺癌细胞侵袭能力的影响，组间比较采用n t检验。n 结果:miR-630在乳头状甲状腺癌组织中的表达水平为(1.13±0.07)，其表达水平明显低于癌旁组织表达水平(5.33±0.08)，差异有统计学意义(n t＝15.472，n P<0.05)；miR-630 mimics上调甲状腺乳头状癌TPC-1细胞中miR-630后，Slug mRNA表达水平(0.48±0.09)明显低于对照组细胞(1.03±0.07，n t＝7.132，n P<0.05)，差异有统计学意义；miR-630 mimics与Slug 3’非翻译区野生型共转染组荧光素酶活性明显低于对照组(0.38±0.03，n t＝13.021，n P0.05)，差异无统计学意义；miR-630 mimics组侵袭细胞数(241.3±42.4)明显低于对照组侵袭细胞数(467.5±51.7)，差异有统计学意义(n t＝3.214，n P0.05)。n 结论:miR-630能够通过靶向抑制Slug的表达，抑制甲状腺癌细胞的侵袭能力。“,”Objective:To observe the effect of microRNA (miRNA, miR)-630 in thyroid cancer (TC) cells on the expression of the epithelial-mesenchymal transition (EMT) marker Slug, and its influence on cell invasion.Methods:A total of 40 cases of papillary TC and their para-cancerous specimens were collected from the Department of General Surgery of Hebei Medical University from January 2018 to December 2019. The real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression level of miR-630 in TC and its adjacent tissues. After transfecting miR-630 mimics into human TC cell line TPC-1, the expression level of Slug gene was detected by Real-time PCR, and the regulatory effect of miR-630 on Slug gene was observed by double luciferase reporter gene assay. The overexpression Slug plasmid and miR-630 mimics were co-transfected into TPC-1 cells. Transwell cell invasion assay was used to observe the effect of miR-630 and Slug expression on the invasion of TC cells.Results:Real-time PCR results showed that the expression level of miR-630 in papillary TC tissues was (1.13±0.07), significantly lower than that in adjacent tissues (5.33±0.08) with the difference being statistically significant (n t=15.472, n P<0.05), and miR-630 mimics up-regulated miR-630 in papillary TC cells, and Slug mRNA expression level (0.48±0.09) was also significantly lower than that in control group (1.03±0.07,n t=7.132, n P<0.05). The luciferase activity of miR-630 mimics and Slug 3′wild-type co-transfection group was significantly lower than that of control group (0.38±0.03,n t=13.021, n P0.05). The number of invasive cells in mir-630 mimics group (241.3±42.4) was significantly less than that in control group (467.5±51.7,n t=3.214, n P0.05).n Conclusion:miR-630 can inhibit the invasion ability of TC cells by targeting Slug.