论文部分内容阅读
目的 :以聚己内酯(polycaprolactone,PCL)为静电纺丝材料,制备载有辛伐他汀(simvastatin,SMV)的纤维膜,分析其药物缓释特征,构建具有SMV缓释功能的支架材料,用于牙周组织工程。方法:将SMV和PCL溶解于三氯甲烷,得到均相溶液,制备载SMV的PCL纤维膜作为实验组,以纯PCL纤维膜作为对照组。对所得纤维膜采用扫描电子显微镜和广角X射线衍射进行形貌和表征分析,通过紫外分光光度计测定纤维膜释放至介质中的SMV,绘制释放曲线,分析载SMV纤维膜的药物缓释特征。采用SPSS 17.0软件包对数据进行统计学分析。结果:SMV/PCL纤维膜由纳米级静电纺丝构成,SMV与PCL结合较好,药物包裹于纤维之中;累计SMV释放量与时间接近线性相关,能够实现药物的长效缓释。结论:通过静电纺丝技术构建SMV/PCL纳米纤维膜,具有良好的药物缓释作用,有望作为支架材料应用于牙周组织工程。
OBJECTIVE: To prepare the fibrin film loaded with simvastatin (SMV) by using polycaprolactone (PCL) as electrospun material, analyze its drug release characteristics and construct a scaffold material with SMV sustained- For periodontal tissue engineering. Methods: SMV and PCL were dissolved in chloroform to obtain a homogeneous solution. PCL fibers loaded with SMV were prepared as experimental groups and pure PCL fibers as control. Morphology and characterization of the obtained membrane were characterized by scanning electron microscopy and wide-angle X-ray diffraction. The release of SMV from the membrane to the medium was measured by UV spectrophotometer. The release curve was drawn and the sustained release characteristics of SMV membrane were analyzed. Data were statistically analyzed using SPSS 17.0 software package. Results: The SMV / PCL membrane was composed of nano-sized electrospun fibers. The combination of SMV and PCL was better and the drug was encapsulated in the fibers. The cumulative release of SMV was linearly correlated with the time, which could prolong the drug release. Conclusion: The SMV / PCL nanofiber membrane prepared by electrospinning technology has good drug release and is expected to be used as a scaffold in periodontal tissue engineering.