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将番茄红果材料‘OH 88119’与棕果材料‘Black Cherry’杂交获得F2分离群体。通过对成熟果实外果皮、中果皮/内果皮和胎座颜色的观察,在不考虑番茄红素颜色的情况下,外果皮和中果皮/内果皮的颜色分别由一个基因控制,且独立遗传。用gf位点特异引物经PCR和RT-PCR扩增和测序,获得了‘Black Cherry’和‘紫色圣女果’的基因组DNA序列以及8个棕果番茄的c DNA序列,与红果材料中对应的GF位点序列相比,‘Black Cherry’、‘黑番茄(9T)-h’、‘黑番茄–2012’和‘紫果(圆)’的编码区出现了一个T与C替换,‘紫果(椭圆)’编码区缺失了两个核苷酸AT,而‘紫色圣女果’、‘紫玉(F1)-h-h’和‘Chocolate Cherry’则在编码区插入了一个A,这些突变分别属于gf 4、gf 3和gf 2类型,都形成新的终止密码子。根据序列差异建立了可用于鉴定gf 3和gf 4的d CAPS(Derived Cleaved Amplified Polymorphic Sequence)标记,为棕果番茄的标记辅助选择提供工具。
F2 segregation population was obtained by crossing tomato fruit material ’OH 88119’ with ’Brown Cherry’. By examining the color of the exocarp, mesocarp / endocarp and placenta of mature fruits, the color of the pericarp and mesocarp / endocarp were independently controlled by one gene independently of the lycopene color. The genomic DNA sequences of ’Black Cherry’ and ’Purple Jojoba’ and the c DNA sequences of eight tomato fruits were obtained by PCR and RT-PCR amplification using gf locus-specific primers and corresponding to the red fruit material A T and C substitution occurred in the coding regions of ’Black Cherry’, ’Black Tomato (9T) -h’, ’Black Tomato-2012’ and ’Purple Fruit’ The ORF (coding region) is missing two nucleotides, AT, whereas ’purple sago fruit’, ’purple safflower (F1) -h-h’ and ’Chocolate Cherry’ insert an A in the coding region The mutations belong to the types gf 4, gf 3 and gf 2, respectively, and form new stop codons. Based on the sequence differences, a d CAPS (Derived Cleaved Amplified Polymorphic Sequence) marker was established for the identification of gf 3 and gf 4, providing a tool for marker-assisted selection of palm tomato.