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目的研究重组腺病毒Ad-hIL-24对A549肺癌细胞的影响。方法将载有hIL-24基因的复制缺陷型腺病毒载体(Ad-hIL-24)感染A549肺癌细胞,RT-PCR法检测hIL-24基因的表达,光学显微镜及荧光显微镜下观察病毒感染前后细胞形态的变化,运用MTT法和流式细胞术(FCM)分别检测其对A549细胞的生长抑制及凋亡效应,通过免疫细胞化学分析caspase-3的变化。结果Ad-hIL-24感染A549细胞后有hIL-24目的基因的转录,可明显抑制A549细胞的生长,凋亡率明显增加,并能上调caspase-3的表达。结论腺病毒介导的hIL-24基因具有抑制A549肺癌细胞生长和诱导细胞凋亡的作用。
Objective To study the effect of recombinant adenovirus Ad-hIL-24 on A549 lung cancer cells. Methods Human lung adenocarcinoma A549 cells were transfected with replication-defective adenovirus vector (Ad-hIL-24) containing hIL-24 gene. The expression of hIL-24 gene was detected by RT-PCR. The cells were observed under light and light microscope Morphological changes were detected by MTT assay and flow cytometry (FCM) were used to detect the growth inhibition and apoptosis of A549 cells. The changes of caspase-3 were analyzed by immunocytochemistry. Results The transcription of hIL-24 gene after A549 cells was infected with Ad-hIL-24 significantly inhibited the growth of A549 cells. The apoptosis rate was significantly increased and the expression of caspase-3 was up-regulated. Conclusion Adenovirus-mediated hIL-24 gene can inhibit A549 lung cancer cell growth and induce apoptosis.