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对轮作(前3 a均种植玉米)和连作(连续4 a种植马铃薯)条件下马铃薯“大西洋”品种出苗期和落花期根区土壤溶液进行GC-MS测定,研究连作逆境下马铃薯根系分泌物的自毒物质。结果发现,两种根区土壤溶液中匹配度80%以上的有机物均检测出烃、苯、酸、醇、酯、醛、酮,酰胺及其烃衍生物,其中出苗期有13种有机物是连作土壤溶液中独有物质,且含量最高的是丙酸丙酯(22.335%),最低的是2-十一烯醛(0.258%)。落花期连作土壤溶液中新增有机化合物7种,其中十六烷酸丙基酯也称棕榈酸丙基酯是植物自毒物质。比对出苗期和落花期两种土壤溶液有机物,确定12种化合物〔壬烷、丙酸丙酯、辛醛、二丁基羟基甲苯、1,2-苯二甲酸二(2-甲基丙基)酯、2,6-二甲基壬烷、2-辛烯醛、2-十一烯醛、1-十二烯、1-十三烷醇、1-十五碳烯、5-十八烯〕不是马铃薯自毒化合物。落花期连作土壤溶液中独有的N,N-二甲基甲酰胺化合物也是出苗期连作土壤独有物质,其浓度为0.455%(出苗期)和0.699%(落花期),该物质是否为自毒物质尚需进一步研究。
The root-zone soil solution at the seedling stage and the inflorescence stage of potato / “Atlantic” under rotation (the first 3 years of planting maize) and continuous cropping (continuous 4 years of planting potato) were analyzed by GC-MS to study the root exudation Self-toxic substance. The results showed that hydrocarbons, benzene, acids, alcohols, esters, aldehydes, ketones, amides and their hydrocarbon derivatives were detected in organic matter with the matching degree of more than 80% in the two kinds of soil solutions. Among them, 13 kinds of organic compounds in the seedling stage were continuous cropping The only substance in soil solution, and the highest content of propyl propionate (22.335%), the lowest is 2-undecenoic acid (0.258%). There are 7 kinds of organic compounds added in the soil solution during the flowering period, of which propyl palmitate is also called as autotoxicity substance. Comparing the organic matter in two kinds of soil solution at the seedling stage and the flowering stage, 12 kinds of compounds [nonane, propyl propionate, octylaldehyde, dibutylhydroxytoluene, bis (2-methylpropyl) ) Esters, 2,6-dimethyl nonane, 2-octenal, 2-undecenal, 1-dodecene, 1-tridecanol, 1-pentadecene, 5- Ene] is not a potato autotoxic compound. The unique N, N-dimethylformamide compound in the soil solution during the flowering stage was also the unique soil-growing continuous crop, with the concentration of 0.455% (seedling emergence) and 0.699% (flowering stage) Poisonous substances need further study.