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本文报导CEA单抗的制备及免疫学性状。免疫用纯化的CEA抗原为从结肠癌肝转移灶提取。20μg/0.1ml纯化抗原免疫BALB/C小鼠。取免疫脾脏细胞上骨髓瘤细胞SP2/0融合,经HAT培养基选择,用ELISA法进行筛选。筛选时先用免抗CEA
This article reports the preparation and immunological properties of CEA monoclonal antibodies. The immunization with purified CEA antigen was extracted from liver metastases of colon cancer. BALB/C mice were immunized with 20 μg/0.1 ml purified antigen. The SP2/0 fusion of myeloma cells on immune spleen cells was selected and selected by HAT medium and screened by ELISA. Use anti-CEA first when screening