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为研究蜡梅SAMT基因的调控功能,通过构建pBI121-CpSAMT植物双元表达载体,利用农杆菌介导法将其转入矮牵牛中,经PCR和RT-PCR对转化植株进行检测,同时采用顶空固相微萃取以及气相色谱-质谱技术(HS-SPME-GC-MS),对转基因矮牵牛鲜花进行花香成分分析。结果显示:9株转化苗均能扩增出目的条带,RT-PCR检测结果进一步证明,阳性植株均发生了正确转录;转基因矮牵牛植株和未转基因对照植株在植株大小、叶片形态、花色、花瓣大小以及花期等方面均未发现有明显差异。鲜花芳香成分分析表明,转CpSAMT基因矮牵牛中苯甲醛和苯乙醇含量明显升高,并产生了香茅醇、乙酸香茅酯和乙酸苯乙酯等成分,但水杨酸甲酯和苯甲酸甲酯含量没有显著改变。
In order to study the regulation function of SAMT gene in Chimonanthus praecox, the binary vector pBI121-CpSAMT was constructed and transformed into petunia by Agrobacterium-mediated transformation. The transformed plants were detected by PCR and RT-PCR. Headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) were used to analyze floral components of transgenic petunia flowers. The results showed that all the 9 transformed seedlings could amplify the target bands. RT-PCR results further confirmed that the positive plants were correctly transcribed. The transgenic plantlets and non-transgenic control plants had the highest plant size, leaf morphology, , Petal size and flowering were found no significant differences. Analysis of aromatic components of flowers showed that the contents of benzaldehyde and phenylethyl alcohol in petunia transferred to CpG AMT gene were significantly increased, and components like citronellol, citronellyl acetate and phenethyl acetate were produced, but methyl salicylate and benzene The methyl formate content did not change significantly.