目的：建立人肝微粒体中尼莫地平及其代谢物的HPLC测定法，并用本法研究尼莫地平在微粒体中代谢的动力学。方法：采用 HPLC 法，色谱柱为 Hypersil C１８柱，流动相为乙腈-水(62∶38)检测波长 UV 238 nm，样品用乙醚-正已烷(1∶1)提取。结果：本法的回收率94.3%～98.5%，日内和 OBJECTIVE: To establish an HPLC method for the determination of nimodipine and its metabolites in human liver microsomes. This method was used to study the kinetics of nimodipine metabolism in microsomes. Methods: The HPLC method was used on a Hypersil C18 column with acetonitrile-water (62:38) UV detection at a wavelength of 238 nm. The sample was extracted with diethyl ether-n-hexane (1: 1). Results: The recovery rate of this law 94.3% ~ 98.5%, days and days