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背景与目的所谓肺硬化性血管瘤(so-called pulmonary scleraosing hemangioma,PSH)组织中两种形态不同的主要细胞--间质内多角形细胞和表面立方细胞的某些基因的表达是否相同尚不清楚。揭示他们的基因表达异同点,对于理解两种细胞的分化状态、组织发生以及对该肿瘤进行诊断和鉴别诊断具有重要的意义。方法应用激光捕获显微切割技术(laser capture microdissection,LCM)获取PSH组织中多角形细胞和表面立方细胞,分别提取总RNA,应用RT-PCR方法分别检测形态上完全不同的两种细胞的角蛋白(cytokeratin,CK)、上皮膜抗原(epitheliem membrane antigen,EMA)、波形蛋白(vi mentin,VT)、表面蛋白(surfactant protein B,SP-B)、甲状腺转录因子1(thyroid transcription factor-1,TTF-1)、突触素(synaptophys-in,Syn)及嗜铬素(chromogranin A,ChA)的mRNA表达。结果表面立方细胞具有明确的CK、EMA和SP-B的基因表达,间质内多角形细胞则有明确的VT、Syn和弱的EMA表达,而TTF-1在两者中都有表达,ChA在二者中都无表达。结论PSH组织中的多角形细胞和表面立方细胞在形态表型上的差异可能是由于所处不同的分化状态所致,表面立方细胞趋向肺泡Ⅱ型细胞分化,而间质内的多角形细胞则具有多向分化的潜能。
BACKGROUND & OBJECTIVE: The expression of two genes of two different morphologies, so-called pulmonary scleraosing hemangioma (PSH), is the same for some genes in interstitial polygonal cells and surface cubic cells. clear. Revealing the similarities and differences of their gene expression is of great significance for understanding the differentiation status of the two kinds of cells, the occurrence of the tissue and the diagnosis and differential diagnosis of the tumor. Methods The polygonal cells and surface cubic cells in PSH were obtained by laser capture microdissection (LCM). The total RNA was extracted respectively. The keratin of two morphologically distinct cells were detected by RT-PCR. cytokeratin (CK), epitheliem membrane antigen (EMA), vimentin (VT), surfactant protein B (SP-B), thyroid transcription factor-1 -1), Synaptophysin (Syn) and chromogranin A (ChA) mRNA expression. Results The surface cells showed definite gene expression of CK, EMA and SP-B, interstitial polygonal cells showed definite VT, Syn and weak EMA expression, while TTF-1 was expressed in both, ChA There is no expression in either. Conclusion The morphological differences between polygonal cells and surface cubic cells in PSH may be attributed to different differentiation states. The surface cubic cells tend to differentiate into alveolar type Ⅱ cells while the interstitial polygonal cells Has the potential of multi-directional differentiation.