分别应用单克隆抗BrdU和~3H胸腺嘧啶标记DNA方法检测了表皮生长因子对肝细胞DNA合成的影响,并对两种方法进行了比较。结果显示,两种方法均证明EGF具有明显的刺激肝细胞DNA合成的作用。单克隆抗BrdU标记DNA方法较之~3H胸腺嘧啶法更特异、准确,可以清楚地区分DNA合成期与非合成期细胞。同时简便、完全和省时。 The effects of epidermal growth factor on hepatocyte DNA synthesis were tested using monoclonal anti-BrdU and ~3H thymidine labeled DNA methods, respectively, and the two methods were compared. The results showed that both methods demonstrated that EGF has a significant role in stimulating DNA synthesis in hepatocytes. The monoclonal anti-BrdU-labeled DNA method is more specific and accurate than the ~3H thymidine method and can clearly distinguish DNA synthesis and non-synthetic cells. Simultaneously simple, complete and time-saving.