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应用间接血凝试验(IHAT)和间接荧光抗体试验(IFAT)对30例包虫病患者的血清进行了检测,抗体阳性率分别为86.7%和90.0%,而肝囊肿、肝脓肿、肿瘤和旋毛虫病患者及健康人血清均为阴性反应,囊虫病患者的交叉反应率分别为37.5%和31.3%。包虫囊肿的部位、数量及囊肿是否破裂对检测结果有明显影响。包虫IHAT冻干抗原和包虫囊壁冰冻切片抗原在-20℃可分别保存2a和3个月而不丧失活性。结果表明:IHAT和IFAT对人体包虫病的诊断具有较高的敏感性和特异性。
Serum samples from 30 patients with echinococcosis were detected by indirect hemagglutination test (IHAT) and indirect immunofluorescence test (IFAT). The antibody positive rates were 86.7% and 90.0%, respectively. However, hepatic cysts, liver abscesses , Tumor and Trichinellosis patients and healthy human serum were negative response, Cysticercosis patients cross-reactivity rates were 37.5% and 31.3%. Hydatid cyst site, the number and rupture of the cyst has a significant impact on test results. Hydatid cyst IHAT lyophilized antigen and hydatid cyst wall frozen section antigen can be stored at -20 ℃ for 2 and 3 months respectively without loss of activity. The results show that IHAT and IFAT have high sensitivity and specificity for the diagnosis of human echinococcosis.