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目的 研究阴道毛滴虫与生殖道上皮的关系 ,探讨虫体的致病机制。 方法 应用透射与扫描电镜技术 ,结合光镜免疫组织化学等技术 ,观察在动物模型中虫体对大鼠阴道粘膜的粘附作用。 结果 虫体为PAS阳性 ,成群虫体粘附于阴道的中上段表面的富含粘多糖棱柱状上皮细胞 ;虫体组织蛋白酶阳性 ,常见释放水解酶破坏上皮细胞膜 ;虫体肌动蛋白阳性 ,显示微丝束在阿米巴样虫体内呈网络状。虫体可呈阿米巴样穿钻于上皮细胞间 ,其微丝状伪足可伸入上皮细胞微绒毛间包绕蚕食微绒毛 ,其指状伪足可插入上皮细胞间包绕局部。少量虫体附着在阴道下段粘膜褶皱间的角化上皮。 结论 虫体嗜好寄生阴道穹窿等处 ,是因表层上皮细胞内富含粘原颗粒 ,表面有较多微绒毛。虫体在粘附后释放水解酶消化并吞噬上皮细胞 ,可直接损伤生殖道寄生部位的上皮 ,且大量摄取粘多糖 ,影响阴道自洁 ,继而引起组织炎症。虫体的细胞骨架、细胞衣、多形性伪足和溶酶体 ,分别在变形、粘附、包绕、吞噬与消化过程中起重要作用。
Objective To study the relationship between Trichomonas vaginalis and reproductive tract epithelium and to explore the pathogenesis of parasites. Methods Transmission and scanning electron microscopy techniques, combined with light microscopy immunohistochemistry and other techniques, observed in the animal model of parasites in rats vaginal mucosa adhesion. Results The parasites were positive for PAS, and the group of parasites adhered to the vaginal upper surface of the vaginal polyglutamine-rich prismatic epithelial cells. The larvae were positive for cathepsin and the common release of hydrolases destroyed the epithelial cell membrane. The actin- Microfilament showed a network of amoeba-like worms. Insect may be amoeba-like drilling through the epithelial cells, the microfilament pseudopodia can be stretched into the epithelial cells surrounded by microvilli microvilli, the finger pseudopodia can be inserted between the epithelial cells around the local. A small amount of parasites attached to the vaginal mucosal folds between the keratinized epithelium. Conclusions Parasitic parasites such as parasitic vaginal fornix and other places, because the surface epithelial cells are rich in sticky particles, the surface has more microvilli. The parasites release the hydrolytic enzyme to digest and phagocytize the epithelial cells after adhesion, which can directly damage the epithelium of the genital tract parasitic sites, and ingest mucopolysaccharide in large quantities, affecting vaginal self-cleaning and then causing tissue inflammation. The cytoskeleton, cell coat, polymorphic pseudopodia and lysosomes play an important role in the processes of deformation, adhesion, entrapment, phagocytosis and digestion, respectively.