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为挖掘红腺忍冬绿原酸生物合成的关键酶基因,应用生物信息学方法从红腺忍冬基因组数据库中筛选出4个新基因LHPAL4,LHHCT1,LHHCT2,LHHCT3,并预测其编码蛋白的结构和功能。序列分析结果表明,红腺忍冬LHPAL4与LHPAL1关系密切;LHHCT1,LHHCT3,LHHQT2关系密切,LHHCT2单独聚为一类。通过Real-time PCR检测4个新基因在红腺忍冬不同组织中的转录情况,发现LHPAL4,LHHCT1,LHHCT3在败花中表达量最高,LHHCT2在叶中的表达量最高,为揭示不同部位活性成分含量差异机制提供依据,也为活性成分的合成生物学提供元件。
In order to find out the key enzyme genes involved in the biosynthesis of Lonicera chongqing, four new genes, LHPAL4, LHHCT1, LHHCT2 and LHHCT3, were screened from the genome of Lonicera rhizogenes using bioinformatics methods and the structure and function of the encoded proteins were predicted . Sequence analysis showed that LHPAL4 was closely related to LHPAL1, LHHCT1, LHHCT3 and LHHQT2 were closely related, and LHHCT2 clustered together. Real-time PCR was used to detect the transcriptional status of four new genes in different tissues of Lonicera chingii. LHPAL4, LHHCT1 and LHHCT3 were found to be the most abundant in litchi and LHHCT2 was the most abundant in leaf, Content difference mechanism to provide the basis, but also for the active component of the synthetic biology to provide components.