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为评价一种可触摸等离子体的消毒灭菌效果,利用自制微秒脉冲电源和等离子体反应器,产生了面积为459 cm2的常压表面介质阻挡放电(SDBD)等离子体,以琼脂平板表面的细菌为处理对象,研究了等离子体的消毒灭菌过程。研究结果表明:等离子体的灭菌过程主要发生在前5~10 s,初始细菌数量约为104 CFU时,大肠杆菌、金黄色葡萄球菌、白色葡萄球菌和铜绿假单胞菌都被杀灭2.5~2.8个对数;初始细菌数量低于600 CFU时,等离子体处理10 s后细菌被全部杀光;等离子体的消毒灭菌效率随着处理间距的增大而降低,当处理间距由1 cm增大到4 cm时,大肠杆菌的杀灭对数值由2.7下降到1.1。因此,等离子体可在10 s内快速有效的杀灭琼脂平板上的试验菌。
To evaluate the sterilizing and sterilizing effect of a touchable plasma, an atmospheric pressure surface dielectric barrier discharge (SDBD) plasma with an area of 459 cm2 was generated using a home-made microsecond pulse power supply and a plasma reactor, Bacteria to deal with the object, the study of the plasma sterilization process. The results showed that the sterilization process of plasma mainly occurred in the first 5 ~ 10 s, and the number of initial bacteria was about 104 CFU, Escherichia coli, Staphylococcus aureus, Staphylococcus aureus and Pseudomonas aeruginosa were killed 2.5 ~ 2.8 logarithm. When the number of initial bacteria was less than 600 CFU, the bacteria were completely killed after 10 s of plasma treatment. The disinfection and sterilization efficiency of plasma decreased with the increase of treatment distance. When the treatment distance was 1 cm When increased to 4 cm, the kill logarithm of E. coli decreased from 2.7 to 1.1. Therefore, the plasma can quickly and effectively kill test bacteria on agar plates within 10 s.