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目的测定本市首例人感染禽流感病毒A/Zhejiang/LS01/2014(H7N9)株HA基因序列,分析H7N9禽流感病毒HA基因特点。方法采用real-time PCR检测患者H7N9禽流感病毒核酸,RT-PCR扩增病毒HA基因后测定核苷酸序列。结果实验室确诊丽水首例人感染H7N9禽流感患者。测序得到H7N9禽流感病毒长度为1 683 bp的HA基因核苷酸全序。A/Zhejiang/LS01/2014(H7N9)与我国江浙沪分离株HA基因核苷酸和氨基酸序列相似性都比较高,分别为98.8%~99.5%、98.9%~99.5%;与浙江鸡分离株A/chicken/Zhejiang/C483/2013(H7N9)序列相似性最高。A/Zhejiang/LS01/2014(H7N9)与我国江浙沪H7N9病毒株HA基因序列均处于同一进化分支;与浙江外环境分离株A/environment/Hangzhou/34/2013(H7N9)进化关系最近;与浙江鸡分离株A/chicken/Zhejiang/C483/2013(H7N9)进化关系次之;与韩国野鸟分离株A/wildbird/Korea/A3/2011(H7N9)处于同一进化分支。结论 A/Zhejiang/LS01/2014(H7N9)HA基因来源于浙江鸡携带的H7亚型流感病毒。HA基因具有低致病性禽流感病毒基因特征,但与人类呼吸道上皮细胞的亲和力增加,更容易感染到人。
Objective To determine the HA gene sequence of the first human being infected with avian influenza virus A / Zhejiang / LS01 / 2014 (H7N9) and analyze the HA gene characteristics of H7N9 bird flu virus. Methods The nucleic acid of H7N9 bird flu virus was detected by real-time PCR. The nucleotide sequence of HA gene was amplified by RT-PCR. Results The laboratory confirmed the first human infection of H7N9 bird flu in Lishui. Sequence analysis of H7N9 avian influenza virus length of 1 683 bp HA gene nucleotide sequence. A / Zhejiang / LS01 / 2014 (H7N9) shared high homology with the nucleotide and amino acid sequences of HA gene of Jiangsu isolates from China, which were 98.8% -99.5% and 98.9% -99.5%, respectively. / chicken / Zhejiang / C483 / 2013 (H7N9) has the highest sequence similarity. A / Zhejiang / LS01 / 2014 (H7N9) is in the same evolutionary branch as the HA gene sequence of Jiangsu, Zhejiang, and Shanghai H7N9 strains. It has the closest phylogenetic relationship with Zhejiang isolates A / environment / Hangzhou / 34/2013 The evolutionary relationship of chicken A / chicken / Zhejiang / C483 / 2013 (H7N9) was the second, and that of South Korea wild bird A / wildbird / Korea / A3 / 2011 (H7N9) was in the same evolutionary branch. Conclusion The A / Zhejiang / LS01 / 2014 (H7N9) HA gene is derived from the H7 subtype influenza virus carried by Zhejiang chicken. The HA gene has the characteristics of a low-pathogenic avian influenza virus but has increased affinity for human respiratory epithelial cells and is more susceptible to human infection.