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目的研究分离自广藿香内生真菌索氏平脐蠕孢Bipolaris sorokiniana A606的活性次级代谢产物的化学结构及其活性。方法运用正相硅胶、C18反相硅胶、Sephadex LH-20凝胶和HPLC等色谱技术对菌株B.sorokiniana A606发酵液的醋酸乙酯萃取物进行分离纯化,并通过各种谱学分析方法进行结构鉴定;采用SRB染色法和滤纸片法分别测定化合物的细胞毒和抗菌活性。结果从B.sorokiniana A606发酵液的醋酸乙酯萃取物中分离得到2个化合物,分别鉴定为(3S*,4a S*,6a S*,12b S*)-3-(2-羟基-2-丙烷基)-6a,12b-二甲基-9-[(2R*,4R*)-2-(4-甲基-3-己酮基)]-1,2,3,4a,5,6-六氢吡喃并[3,2-a]氧杂蒽-8,11-二酮(1)和precochlioquinol D(2)。结论化合物1为新化合物,命名为11-羟基-12,13-去氢旋孢腔菌醌B。化合物2为首次从该属真菌中分离得到。其中化合物1具有细胞毒和抗菌活性。
Objective To study the chemical structure and activity of the active secondary metabolites isolated from the endophytic fungus Bipolaris sorokiniana A606. Methods The ethyl acetate extract from B. sorokiniana A606 fermentation broth was separated and purified by normal phase silica gel, C18 reverse phase silica gel, Sephadex LH-20 gel and HPLC, and the structures were elucidated by various spectroscopic methods The cytotoxic and antibacterial activities of the compounds were determined by SRB staining and filter paper respectively. Results Two compounds were isolated from the ethyl acetate extracts of B. sorokiniana A606 fermentation broth and identified as (3S *, 4aS *, 6aS *, 12bS *) - 3- (2-hydroxy- (2R *, 4R *) - 2- (4-methyl-3- hexanonyl)] - 1,2,3,4a, 5,6 -hexahydropyrano [3,2-a] xanthene-8,11-dione (1) and precochlioquinol D (2). Conclusions Compound 1 is a new compound, named 11-hydroxy-12,13-dehydro-cyclosporine B. Compound 2 was isolated from this genus for the first time. Among them, compound 1 has cytotoxic and antibacterial activity.