本文对乳杆菌ＤＭ８９０９对大肠杆菌粘附阴道上皮细胞的抑制作用进行了研究。采用３Ｈ－ＴｄＲ将由阴道分离的大肠杆菌进行同位素标记。刮取健康妇女阴道上皮细胞与其进行体外粘附试验作为对照组，试验组先行阴道上皮细胞与乳杆菌ＤＭ８９０９粘附后，再加入标记的大肠杆菌。结果显示：试验组阴道上皮细胞粘附的大肠杆菌的ｃｐｍ值明显低于对照组（Ｐ＜０．０５），说明乳杆菌ＤＭ８９０９能有效地抑制大肠杆菌对阴道上皮细胞的粘附。 In this paper, Lactobacillus DM8909 E. coli adhesion to the vaginal epithelial cells were studied. Escherichia coli isolated from the vagina was isotopically labeled with 3H-TdR. Scrape healthy women vaginal epithelial cells with in vitro adhesion test as a control group, the experimental group of vaginal epithelial cells and lactobacillus DM8909 adhesion, then add labeled E. coli. The results showed that the cpm of Escherichia coli adhered to the vaginal epithelial cells of the experimental group was significantly lower than that of the control group (P <0.05), indicating that Lactobacillus DM8909 can effectively inhibit the adhesion of Escherichia coli to vaginal epithelial cells.