我们用肠癌细胞株HT-29为免疫原,通过细胞融合产生了多株杂交瘤细胞,为了选择具有临床治疗潜力的单克隆抗体及了解这些抗体与肿瘤细胞作用的特点。我们研究了三种肠癌单克隆抗体——Ga_3(IgG_(2a));Ab_5(IgM);Cb_5(IgM)在单独存在的情况下对体外生长的肠癌细胞株HRT-18生长的影响,以及在人补体或人外周血淋巴细胞存在 We used the colon cancer cell line HT-29 as an immunogen to produce multiple hybridoma cells by cell fusion, in order to select monoclonal antibodies with clinical therapeutic potential and to understand the characteristics of these antibodies and tumor cells. We investigated the effects of three monoclonal antibody for colorectal cancer, Ga_3 (IgG_(2a)), Ab_5 (IgM), and Cb_5 (IgM), on the growth of HRT-18 colon cancer cell lines grown in vitro. As well as in human complement or human peripheral blood lymphocytes