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目的探讨ＢＡＣＴＥＣ－４６０在结核菌快速培养中的应用。方法应用ＢＡＣＴＥＣ－４６０检测结核杆菌，并与涂片和改良罗氏培养法（Ｌ－Ｊ）进行比较。结果１０６８份临床标本ＢＡＣＴＥＣ培养阳性２６９份（２５．２％），阳性报告时间平均１２．５天，培养污染２７份（２．５％）；ＢＡＣＴＥＣ阳性率比涂片高１８．４％，比Ｌ－Ｊ法高１０．４％，差异有高度显著性（Ｐ＜０．０５）；ＢＡＣＴＥＣ快速药敏试验表明总耐药率为５１．７％，药敏报告时间平均６．２天；快速菌型测试表明，结核分枝杆菌占９７．４％，非结核分枝杆菌占２．６％；ＢＡＣＴＥＣ快速培养报告时间比Ｌ－Ｊ法总计提前４０天以上。结论认为ＢＡＣＴＥＣ法是目前比较理想的一种快速检测结核菌的方法。
Objective To investigate the application of BACTEC-460 in the rapid culture of Mycobacterium tuberculosis. Methods Mycobacterium tuberculosis was detected by BACTEC-460 and compared with smear and modified Roche culture (L-J). Results A total of 1068 clinical specimens were positive for BACTEC, 269 (25.2%) were positive for culture, and the average time for positive report was 12.5 days and 27 (2.5%) were for culture contamination. The positive rate of BACTEC was 18.4% higher than that of smear L-J method was 10.4%, the difference was highly significant (P <0.05); BACTEC rapid drug susceptibility test showed that the total drug resistance rate was 51.7%, the average drug susceptibility reporting time was 6.2 days; fast Bacteriological tests showed that Mycobacterium tuberculosis accounted for 97.4%, non-TB Mycobacterium accounted for 2.6%; BACTEC rapid culture report time than the L-J method total more than 40 days in advance. The conclusion is that the BACTEC method is an ideal method for rapid detection of Mycobacterium tuberculosis.