论文部分内容阅读
目的:探讨H22荷瘤小鼠肿瘤发生后睾丸基因表达谱改变的特征。方法:采用荷瘤小鼠标准化四诊及辨证方法,以及Gene Chip Mouse Exon1.0 STArray等技术,检测H22荷瘤小鼠睾丸组织早期邪毒壅盛证和气虚证、中期阳气虚证、中晚期气阴阳虚证3个阶段4个证候睾丸基因表达谱的差异,重点关注肿瘤发生后一致上调或下调明显且表达量大的基因。结果:肿瘤发生后三个阶段一致上调的基因5个;一致下调的基因12个,其中7个与性激素和生殖有关;早期上调、中期和中晚期下调的基因1个;早期和中期上调、中晚期下调的基因3个。在这些基因中,Saa3在晚期气阴阳虚证表达上调了近60倍,Abpg、Pip、Car6在早期邪毒壅盛证表达上调了40~80倍,值得关注。结论:肿瘤发生后荷瘤小鼠睾丸基因表达的改变以下调为主,性激素合成与生殖受到影响;一些表达显著改变的基因值得进一步研究。
Objective: To investigate the changes of testicular gene expression profiles in H22 tumor-bearing mice after tumorigenesis. Methods: Four diagnostic and diagnostic methods of tumor-bearing mice were used, and Gene Chip Mouse Exon 1.0 STArray and other techniques were used to detect the early pathogenicity and qi deficiency syndrome of testis in H22 tumor-bearing mice. Qi and yin-yang deficiency syndrome in three stages of four syndromes of testicular gene expression profiles of differences, focusing on the tumor after the consistent up-or down-regulated and the expression of large genes. Results: Five genes were up-regulated in three stages after tumorigenesis. Twelve genes were down-regulated, of which seven were related to sex hormones and reproduction. One was up-regulated, one was down-regulated in middle and late stages, Three genes were down-regulated. Among these genes, Saa3 was up-regulated by up to 60-fold in the syndrome of qi-yang and qi deficiency in the late stage, and Abpg, Pip and Car6 were upregulated 40- to 80-fold in the early stage of culturing. CONCLUSION: The changes of testicular gene expression in tumor-bearing mice after tumorigenesis are mainly down-regulated, and the synthesis and reproduction of sex hormones are affected. Some genes that have significant changes in expression deserve further study.