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采用AFLP(Amplified fragment lengthpolymorphism)技术初步分析了采自黑龙江省稻田49个稻瘟病菌的遗传多样性。这些菌株在0.68的相似水平上被划分为6个宗谱,宗谱G1和G3为优势宗谱,均含有17个菌株,各自占菌株总数的35.1%。在0.90相似水平上被划分为48个宗谱,显示丰富的遗传多样性。人工接种结果表明多数稻瘟病菌株具有强或较强致病力,占69.23%。上述结果为了解黑龙江省稻瘟病菌群体结构特征及水稻抗稻瘟病育种提供信息。
Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of 49 M. grisea isolates collected from paddy fields in Heilongjiang Province. These strains were divided into 6 genealogies at a similarity level of 0.68, and genealogies G1 and G3 were dominant genealogies, each containing 17 strains, each accounting for 35.1% of the total number of strains. Divided into 48 genealogies at a similarity of 0.90, showing a rich genetic diversity. The artificial inoculation results showed that most of the strains of Magnaporthe grisea had strong or strong pathogenicity, accounting for 69.23%. The above results provide information for understanding the structural characteristics of rice blast populations and rice blast resistance breeding in Heilongjiang Province.