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目的探讨转化生长因子-β1(TGF-β1)、基质金属蛋白酶-1(MMP-1)及其组织抑制因子-1(TIMP-1)在晚期血吸虫病(晚血)肝组织和血清中的表达及其与肝组织病理学的关系。方法采用免疫组化SP法检测45例晚血(观察组)肝组织中TGF-β1、MMP-1、TIMP-1表达;采用ELISA法检测观察组和30例健康人(对照组)血清TIMP-1、TGF-β1、MMP-1水平。结果观察组肝组织中TGF-β1、TIMP-1表达强度与肝纤维化程度、有无合并HBV感染呈正相关(P均<0.05);而MMP-1无相关性。与对照组比较,观察组血清TGF-β1、TIMP-1水平及TIMP-1/MMP-1值明显增高(P均<0.01),并与肝纤维化程度、免疫组化表达强度呈正相关(P均<0.01),而血清MMP-1差异无统计学意义。结论TGF-β1、MMP-1、TIMP-1与晚血肝纤维化的发生、发展密切相关,三者联合检测可作为晚血患者肝纤维化诊断和疗效评估指标。
Objective To investigate the expression of transforming growth factor-β1 (TGF-β1), matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 in liver tissue and serum of patients with advanced schistosomiasis And its relationship with liver histopathology. Methods The expression of TGF-β1, MMP-1 and TIMP-1 in liver tissues of 45 patients with late-onset blood (observation group) was detected by immunohistochemistry SP method. The levels of TIMP- 1, TGF-β1, MMP-1 levels. Results The expression of TGF-β1 and TIMP-1 in the liver tissue of the observation group was positively correlated with the degree of liver fibrosis and the presence or absence of HBV infection (all P <0.05), while there was no correlation between the expression of TGF-β1 and TIMP-1. Compared with the control group, the serum levels of TGF-β1, TIMP-1 and TIMP-1 / MMP-1 in the observation group were significantly increased (all P <0.01), and positively correlated with the degree of liver fibrosis and the expression of immunohistochemistry All <0.01), while the serum MMP-1 had no significant difference. Conclusion The expressions of TGF-β1, MMP-1 and TIMP-1 are closely related to the occurrence and development of late-onset hepatic fibrosis. The combined detection of TGF-β1, MMP-1 and TIMP-1 can be used as a diagnostic and therapeutic index for the diagnosis of hepatic fibrosis in patients with late-onset blood.