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目的探讨SALL4基因在宫颈癌组织中的表达及意义。方法收集31例宫颈癌组织和17例正常宫颈组织,用RT-PCR检测SALL4mRNA的表达,用Western blot在蛋白水平上进行验证。分析SALL4在宫颈癌中的表达水平与临床病理因素的相关性。结果宫颈癌组织中SALL4表达高于正常宫颈组织(P<0.01);Western blot在蛋白水平上证实了这一点。宫颈癌组织中SALL4的表达与肿瘤分化状态密切相关(P<0.05),与年龄、FIGO分期、肿瘤大小、病理类型和淋巴结是否转移无明显关系。结论宫颈癌组织中SALL4mRNA的表达上调,且与宫颈癌的分化相关。SALL4的基因和蛋白水平检测可能成为宫颈癌诊断和预后监测的新指标。
Objective To investigate the expression of SALL4 gene in cervical cancer and its significance. Methods Totally 31 cervical cancer tissues and 17 normal cervical tissues were collected. The expression of SALL4 mRNA was detected by RT-PCR and verified by Western blot at the protein level. To analyze the correlation between SALL4 expression and clinicopathological factors in cervical cancer. Results The expression of SALL4 in cervical cancer tissues was higher than that in normal cervical tissues (P <0.01). Western blot confirmed this at the protein level. The expression of SALL4 in cervical cancer was closely related to the differentiation (P <0.05), but not to age, FIGO stage, tumor size, pathological type and lymph node metastasis. Conclusion The expression of SALL4 mRNA in cervical cancer is up-regulated, which is related to the differentiation of cervical cancer. SALL4 gene and protein levels may become a new indicator of cervical cancer diagnosis and prognosis.