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目的探讨反复+GZ暴露对脑的病理生理影响及其机制。方法18只雄性SD大鼠随机分为对照组、+GZ重复暴露后1h组和6h组三组,每组6只。实验组大鼠在动物离心机上经历3次+10GZ/3min(两次之间间歇30min)作用。对照组大鼠G值为+1GZ。分别于暴露后1h及6h将大鼠麻醉后原位固定,完整取脑,利用NADPH-d组织化学方法,观察大鼠脑组织一氧化氮合酶(NOS)阳性神经元分布与染色强度的变化。结果NOS阳性神经元在脑内的一些特殊核团出现。+GZ重复暴露3次后大鼠脑组织NOS阳性神经元及纤维的形态特点与对照组相似。暴露后1h,大鼠顶叶皮层、海马CA1区及纹状体内的NOS阳性神经元数目较对照组分别增加30.0%、250.0%及45.0%(P<0.05);暴露后6h,顶叶皮层、海马CA1区及纹状体内的NOS阳性神经元数目则分别增加30.0%、300.0%及55.0%(P<0.05)。梨状皮层内NOS阳性神经元数目有增加趋势,但与对照组比较未达到显著性水平。上述区域内NOS阳性神经元的灰度较对照组均无显著性差异。结论+10GZ/3min重复暴露3次可引起大鼠顶叶皮层、海马及纹状体等部位NOS阳性神经元数目显著增多?
Objective To investigate the effect of repeated + GZ exposure on the pathophysiology of the brain and its mechanism. Methods Eighteen male Sprague-Dawley rats were randomly divided into control group, 1 hour group with + GZ repeated exposure and 6 hours group with 6 rats in each group. Rats in the experimental group experienced 3 + 10 GZ / 3 min on an animal centrifuge (intermittent 30 min between two times). The control group rats G value of + 1GZ. The rats were fixed in situ after anesthesia at 1h and 6h after exposure respectively, and the changes of distribution and intensity of nitric oxide synthase (NOS) positive neurons in brain tissue were observed by NADPH-d histochemical method . Results NOS positive neurons in the brain of some special nuclei appear. + GZ repeated exposure 3 times after the rat brain tissue NOS positive neurons and fibers morphological characteristics similar to the control group. At 1 hour after exposure, the number of NOS positive neurons in the parietal cortex, hippocampal CA1 region and striatum increased by 30.0%, 250.0% and 45.0%, respectively (P <0.05) compared with the control group. Six hours after exposure, the number of NOS positive neurons in parietal cortex, hippocampal CA1 region and striatum increased by 30.0%, 300.0% and 55.0%, respectively (P <0.05). The number of NOS-positive neurons in piriform cortex increased, but did not reach the significant level compared with the control group. The gray level of NOS positive neurons in the above regions had no significant difference compared with the control group. Conclusion The number of NOS positive neurons in the parietal cortex, hippocampus and striatum increased significantly after repeated exposure of 10GZ / 3min for 3 times.