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目的探讨反复＋ＧＺ暴露对脑的病理生理影响及其机制。方法１８只雄性ＳＤ大鼠随机分为对照组、＋ＧＺ重复暴露后１ｈ组和６ｈ组三组，每组６只。实验组大鼠在动物离心机上经历３次＋１０ＧＺ／３ｍｉｎ（两次之间间歇３０ｍｉｎ）作用。对照组大鼠Ｇ值为＋１ＧＺ。分别于暴露后１ｈ及６ｈ将大鼠麻醉后原位固定，完整取脑，利用ＮＡＤＰＨ－ｄ组织化学方法，观察大鼠脑组织一氧化氮合酶（ＮＯＳ）阳性神经元分布与染色强度的变化。结果ＮＯＳ阳性神经元在脑内的一些特殊核团出现。＋ＧＺ重复暴露３次后大鼠脑组织ＮＯＳ阳性神经元及纤维的形态特点与对照组相似。暴露后１ｈ，大鼠顶叶皮层、海马ＣＡ１区及纹状体内的ＮＯＳ阳性神经元数目较对照组分别增加３０．０％、２５０．０％及４５．０％（Ｐ＜０．０５）；暴露后６ｈ，顶叶皮层、海马ＣＡ１区及纹状体内的ＮＯＳ阳性神经元数目则分别增加３０．０％、３００．０％及５５．０％（Ｐ＜０．０５）。梨状皮层内ＮＯＳ阳性神经元数目有增加趋势，但与对照组比较未达到显著性水平。上述区域内ＮＯＳ阳性神经元的灰度较对照组均无显著性差异。结论＋１０ＧＺ／３ｍｉｎ重复暴露３次可引起大鼠顶叶皮层、海马及纹状体等部位ＮＯＳ阳性神经元数目显著增多?
Objective To investigate the effect of repeated + GZ exposure on the pathophysiology of the brain and its mechanism. Methods Eighteen male Sprague-Dawley rats were randomly divided into control group, 1 hour group with + GZ repeated exposure and 6 hours group with 6 rats in each group. Rats in the experimental group experienced 3 + 10 GZ / 3 min on an animal centrifuge (intermittent 30 min between two times). The control group rats G value of + 1GZ. The rats were fixed in situ after anesthesia at 1h and 6h after exposure respectively, and the changes of distribution and intensity of nitric oxide synthase (NOS) positive neurons in brain tissue were observed by NADPH-d histochemical method . Results NOS positive neurons in the brain of some special nuclei appear. + GZ repeated exposure 3 times after the rat brain tissue NOS positive neurons and fibers morphological characteristics similar to the control group. At 1 hour after exposure, the number of NOS positive neurons in the parietal cortex, hippocampal CA1 region and striatum increased by 30.0%, 250.0% and 45.0%, respectively (P <0.05) compared with the control group. Six hours after exposure, the number of NOS positive neurons in parietal cortex, hippocampal CA1 region and striatum increased by 30.0%, 300.0% and 55.0%, respectively (P <0.05). The number of NOS-positive neurons in piriform cortex increased, but did not reach the significant level compared with the control group. The gray level of NOS positive neurons in the above regions had no significant difference compared with the control group. Conclusion The number of NOS positive neurons in the parietal cortex, hippocampus and striatum increased significantly after repeated exposure of 10GZ / 3min for 3 times.