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目的:研究神经肽ZNC(C)PR通过C6细胞发挥的神经营养作用和机制。方法:以ZNC(C)PR处理C6细胞的条件培养液对PC12细胞生长的影响来观察该肽的间接营养作用,并用受体结合分析来确证该肽在此系统中作用的靶细胞类型。结果:ZNC(C)PR处理的C6细胞的条件培养液能够促进PC12细胞由嗜铬瘤细胞向交感神经元转化;放射性配体分析进一步指出在C6细胞上存在着该肽的特异性结合位点。结论:ZNC(C)PR能够通过位于C6细胞上的受体促进该细胞分泌某种营养性因子,通过这种因子促进PC12细胞的发育。
AIM: To investigate the neurotrophic effect and mechanism of neuropeptide ZNC (C) PR through C6 cells. Methods: The effect of conditioned medium from C6 cells treated with ZNC (C) PR on the growth of PC12 cells was observed to observe the indirect nutrient effect of this peptide and the receptor binding assay was used to confirm the target cell type for this peptide to function in this system. RESULTS: The conditioned medium of C6 cells treated with ZNC (C) PR promoted the transformation of PC12 cells from the pheochromocytoma cells to the sympathetic neurons; radioligand analysis further indicated that there was a specific binding site for this peptide on C6 cells . Conclusion: ZNC (C) PR can promote the secretion of certain nutritional factors through the receptors on C6 cells, and promote the development of PC12 cells through this factor.