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目的 研究乌苏里藜芦生物碱 ( Vn A)对大鼠的抗血栓作用。方法 分别应用电刺激诱发大鼠颈总动脉血栓形成模型和瘀血诱发下腔静脉血栓形成模型评价 Vn A的抗动、静脉血栓形成作用。结果 大鼠 iv6种不同剂量的 Vn A ( 7.2～ 4 2 .9μg/ kg)导致血管阻塞时间 ( OT)显著延长 ,且具明显的剂量依赖性 ,Vn A 30μg/ kg产生的抗动脉血栓形成作用与赖氨匹林 18.0 mg/ kg的作用相当。单次 iv Vn A30 μg/ kg使 OT呈时间依赖性延长。Vn A抗动脉血栓形成作用起效迅速 ,持续至少 80 min,iv后 15 m in达效应峰值。iv Vn A( 15～ 4 5 μg/ kg)显著减少下腔静脉血栓干质量 ,并呈剂量依赖性。结论 Vn A具有强大的抗动、静脉血栓形成作用 ,并具明显的剂量依赖性和时间依赖性。其抗血栓效能高 ,作用强度大 ,在大鼠的有效剂量低至μg/ kg水平。Vn A抗血栓活性的发现为其研究开发展示了新的前景
Objective To study the antithrombotic effects of V. arvensis alkaloids (Vn A) on rats. Methods The rat model of common carotid artery thrombosis induced by electrical stimulation and the model of inferior vena cava thrombosis induced by blood stasis were used to evaluate the antithrombotic and antithrombotic effects of VnA. RESULTS: Vn A (7.2 to 42. 9 μg/kg) at different doses of iv led to a significant increase in vascular occlusion time (OT) in a dose-dependent manner, and Vn A 30 μg/kg produced anti-arterial thrombosis. The effect is comparable to that of lisaplin 18.0 mg/kg. A single iv Vn A30 μg/kg resulted in a time-dependent extension of OT. The Vn A antithrombotic effect was rapid, lasting at least 80 min, and peaked at 15 min after iv. Iv Vn A (15 to 45 μg/kg) significantly reduced the thrombus mass in the inferior vena cava and was dose-dependent. Conclusion Vn A has a strong anti-vein and venous thrombosis with a dose-dependent and time-dependent manner. Its antithrombotic efficacy is high and its effect is high. The effective dose in rats is as low as μg/kg. The discovery of Vn A antithrombotic activity shows new prospects for its research and development