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在干旱胁迫伴随大气CO2浓度以及升高的CO2浓度(加倍)条件下,以过量表达番茄类囊体膜抗坏血酸过氧化物酶基因(StAPX)的转基因番茄为试材,探明干旱胁迫下CO2浓度升高对转基因及其野生型番茄植株清除活性氧及耐旱能力的影响。结果表明:升高的CO2浓度明显增加了干旱胁迫下植物的光合水平;升高的CO2浓度明显降低了干旱导致的植物体内H2O2和O2ˉ的积累,影响了干旱胁迫下番茄植株的水-水循环系统的活性氧清除酶活性和小分子抗氧化物质含量;干旱胁迫下即使伴随升高的CO2浓度,测试番茄植株体内的渗透调节物质含量变化也不太明显;升高的CO2浓度明显降低了干旱胁迫下的植物细胞膜伤害程度;干旱胁迫下,升高的CO2浓度对转基因番茄株系比对野生型植株的影响更加明显。结果证明干旱逆境下,升高的CO2浓度能够在一定程度上进一步提高转基因番茄植株的耐旱性。
Under drought stress accompanied by atmospheric CO2 concentration and elevated CO2 concentration (doubling), transgenic tomato over-expressing tomato asparaginase gene (StAPX) was used to study the effects of drought stress on CO2 concentration Effects of Elevation on Scavenging Reactive Oxygen and Drought Tolerance in Transgenic and Wild-type Tomato Plants. The results showed that elevated CO2 significantly increased the photosynthesis of plants under drought stress; elevated CO2 concentration significantly reduced the accumulation of H2O2 and O2ˉ in plants induced by drought, and affected the water-water circulation system of tomato plants under drought stress Activity of oxygen scavenging enzyme and content of small molecule antioxidants. Under the drought stress, the content of osmotic adjusting substances in the tested tomato plants was not significantly changed even with the elevated CO2 concentration. The elevated CO2 concentration significantly reduced the drought stress Under the drought stress, the elevated CO2 concentration had more obvious effect on the transgenic tomato lines than the wild-type plants. The results show that under drought stress, elevated CO2 concentration can further improve the drought tolerance of transgenic tomato plants to a certain extent.