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Hepatitis B virus surface antigen (HBsAg) inRNA has been enriched from a hepatoma cell line (PLC/PRF/5) by specific polysome immunoprecipitation and used for cDNA cloning. A HBsAg cDNA clone was identified by in situ hybridization with a cloned viral probe. It was characterized by restriction endonuclease mapping and DNA sequence analysis. Molecular hybridization of PLC/PRF/5 cellular DNA and RNA to [~(32)P]-labeled HBsAg cDNA revealed the integration of at least six copies of the hepatitis B virus (HBV) DNA into the host genome and expression of three DNA species confining HBsAg-specific sequences. The possible role of HBV in the oncogenesis of primary hepatocellular carcinoma is discussed.
Hepatitis B virus surface antigen (HBsAg) inRNA has been enriched from a hepatoma cell line (PLC / PRF / 5) by specific polysome immunoprecipitation and used for cDNA cloning. A HBsAg cDNA clone was identified by in situ hybridization with a cloned viral probe. Molecular was characterized by restriction endonuclease mapping and DNA sequence analysis. Molecular hybridization of PLC / PRF / 5 cellular DNA and RNA to [~ (32) P] -labeled HBsAg cDNA revealed the integration of at least six copies of the hepatitis B virus HBV) into the host genome and expression of three DNA species confining HBsAg-specific sequences. The possible role of HBV in the oncogenesis of primary hepatocellular carcinoma is discussed.