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目的探讨补肾活血中药对肾小球肾炎大鼠肾组织转化生长因子-β1(TGF-β_1)及结缔组织生长因子(CTGF)表达的影响。方法选取6月龄Wister健康雄性大鼠40只,对其中30只大鼠建立肾小球肾炎大鼠模型,并将此30只造模成功的大鼠随机分为模型组、中药组和盐酸贝那普利组,每组10只,选取另10只未建模大鼠作为对照组;对照组和模型组均给予等量生理盐水灌胃,中药组给予补肾活血中药灌胃,盐酸贝那普利组给予西药盐酸贝那普利灌胃,治疗8周后,测定各组24h尿蛋白、血尿素氮和血肌酐水平,处死后采用免疫荧光化学法检测TGF-β_1和CTGF蛋白相对表达量。结果干预过程中模型组有1只大鼠死亡;模型组、中药组和盐酸贝那普利组大鼠24h尿蛋白分别为(96.58±10.32)、(49.76±9.82)和(50.30±10.02)mg/24h,血尿素氮分别为(10.82±0.59)、(5.98±0.57)和(6.03±0.52)mmol/L,血肌酐分别为(33.54±5.32)、(17.89±5.17)和(17.96±5.03)μmol/L,均高于对照组的(10.38±2.17)mg/24h、(4.01±0.37)mmol/L、(2.55±0.71)μmol/L(均P<0.05),且中药组和盐酸贝那普利组24 h尿蛋白、血尿素氮和血肌酐水平均低于模型组(均P<0.05),而中药组和盐酸贝那普利组各指标水平差异均无统计学意义(均P>0.05);模型组、中药组和盐酸贝那普利组大鼠TGF-β_1免疫荧光相对含量分别为(5.296±0.124)、(0.568±0.156)和(1.697±0.468),CTGF免疫荧光相对含量分别为(5.347±1.285)、(1.485±0.466)和(1.078±0.327),均高于对照组的(0.035±0.007)和(0.017±0.004)(均P<0.05),且中药组和盐酸贝那普利组TGF-β_1和CTGF免疫荧光相对含量均低于模型组(均P<0.05),中药组TGF-β_1免疫荧光相对含量低于盐酸贝那普利组(t=7.237,P<0.001),而2组CTGF免疫荧光相对含量差异无统计学意义(P>0.05)。结论补肾活血中药可明显降低肾小球肾炎大鼠实验室相关指标,抑制TGF-β_1和CTGF表达水平。
Objective To investigate the effect of Bushen Huoxue Chinese medicine on the expression of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) in kidney of rats with glomerulonephritis. Methods Forty male Wistar rats of 6-month-old Wister rats were randomly divided into three groups: model group, Chinese herb group, Nalapril group, 10 rats in each group, and the other 10 non-model rats were selected as the control group; the control group and model group were given the same amount of saline gavage, the Chinese medicine group was given Bushenhuoxue Chinese medicine, After 8 weeks of treatment, the urinary protein, blood urea nitrogen and serum creatinine of each group were measured. The relative expression of TGF-β 1 and CTGF protein were detected by immunofluorescence staining after sacrificed. Results In the intervention group, one rat died in the model group. The 24h urine protein in model group, Chinese herb group and benazepril hydrochloride group were (96.58 ± 10.32), (49.76 ± 9.82) and (50.30 ± 10.02) mg respectively / 24h, and the blood urea nitrogen were (10.82 ± 0.59), (5.98 ± 0.57) and (6.03 ± 0.52) mmol / L respectively, and the serum creatinine were (33.54 ± 5.32), (17.89 ± 5.17) and (17.96 ± 5.03) (4.01 ± 0.37) mmol / L and (2.55 ± 0.71) μmol / L, respectively (all P <0.05). Compared with the control group, 24 h urinary protein, blood urea nitrogen and serum creatinine in Puli group were lower than those in model group (all P <0.05), while there was no significant difference in each index between TCM group and benazepril hydrochloride group (P> 0.05). The relative contents of TGF-β 1 in model group, Chinese herb group and benazepril hydrochloride group were (5.296 ± 0.124), (0.568 ± 0.156) and (1.697 ± 0.468) respectively, and the relative contents of CTGF immunofluorescence Were (5.347 ± 1.285), (1.485 ± 0.466) and (1.078 ± 0.327), respectively, which were significantly higher than those in the control group (0.035 ± 0.007) and (0.017 ± 0.004) The relative content of TGF-β 1 and CTGF immunofluorescence in Pulimil group was lower than that in model group ( (P <0.05). The relative content of TGF-β 1 immunofluorescence in Chinese medicine group was lower than that in benazepril hydrochloride group (t = 7.237, P <0.001), while there was no significant difference in the relative content of CTGF immunofluorescence ). Conclusion Bushen Huoxue Chinese herbs can significantly reduce the glomerulonephritis rat laboratory-related indicators, inhibit TGF-β 1 and CTGF expression levels.