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Our previous studies revealed that second malevibration signal (SMVS) restrained the matingbehavior of N. lugens, the influences of threebiological features (density, age, and wingform) on SMVS’s inhibitory effect were hereinstudied by playing back its record. The dura-tion of playback was 4 h. Except otherwisestatement, N. lugens tested were virginmacropterous males and females aged 4-6 d af-ter emergence, and the density was 5 pairs (5females and 5 males) of N. lugens per cage (4cm in diameter and 8 cm in height). The in-hibitory effect of SMVS was evaluated usingmating rate (i. e. the rate of females withspermatophore). The results were as follows:
The previous of the two malevibration signals (SMVS) restrained the mating behavior of N. lugens, the influences of three biologic features (density, age, and wingform) on SMVS’s inhibitory effect were stamped by playing back its record. The dura tion of playback was 4 h. Except otherwise complete, N. lugens tested were virgin macropterous males and females aged 4-6 d af-ter emergence, and the density was 5 pairs (5 females and 5 males) of N. lugens per cage (4 cm in diameter and 8 cm in height. The in-hibitory effect of SMVS was evaluated usingmating rate (ie the rate of females with spermatophore). The results were as follows: