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建立了高效液相色谱-串联质谱法测定大鼠血浆中的积雪草酸氨丁三醇盐。采用Kromasil C18柱,以乙腈∶0.1%甲酸为流动相,梯度洗脱,以醋酸泼尼松龙为内标,ESI源负离子模式、多反应监测扫描,药物与内标的监测离子对为m/z 533.00→487.25和m/z 447.00→401.15。结果表明,药物在10~1 000 ng/ml浓度范围内线性关系良好,批内和批间RSD均小于10%。低、中、高浓度标准血样的提取回收率分别为77.3%、81.0%和73.9%,RSD分别12.3%、5.3%和3.1%。
A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) method was developed for the determination of asiatic acid tromethamine in rat plasma. Kromasil C18 column with acetonitrile: 0.1% formic acid as mobile phase, gradient elution, prednisolone acetate internal standard, ESI source negative ion mode, multiple reaction monitoring scan, drug and internal standard monitoring ion pair m / z 533.00 → 487.25 and m / z 447.00 → 401.15. The results showed that the drug had a good linearity in the concentration range of 10-1 000 ng / ml, and the intra-assay and inter-assay RSD were less than 10%. The recovery rates of low, middle and high standard blood samples were 77.3%, 81.0% and 73.9%, respectively, with RSDs of 12.3%, 5.3% and 3.1%, respectively.