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目的探讨血清肾酶、两个单核苷酸多态性与妊娠期糖尿病(GDM)的关系。方法选取2013年6月-2015年6月在该院住院或门诊体检的GDM孕妇99例;妊娠期血糖正常孕妇99例(对照组),测量年龄(岁)、体重(kg)、体重指数(BMI)(kg/m~2)、机体脂肪(BF)(%)、妊娠期体重增加(kg)、空腹血糖(mmol/L)、餐后2 h血糖(mmol/L)、血清肾酶(μg/ml)、2型糖尿病(T2DM)家族史阳性率等;采用聚合酶链反应-限制性片段长度多态性方法对DNA样本进行基因分型:单核苷酸多态性rs2576178和rs10887800。结果在年龄匹配的病例对照研究中发现,血清肾酶水平比较,差异无统计学意义(P>0.05);rs10887800基因多态性变异与GDM有一定关系,经过多次调整后差异仍有统计学意义(P<0.05);而rs2576178基因多态性与GDM呈弱相关(P=0.030),经过多次调整后无相关性(P=0.09)。结论 rs10887800多态性与GDM可能具有一定的相关性,rs2576178基因多态性与GDM呈弱相关性。
Objective To investigate the relationship between serum renal enzyme, two single nucleotide polymorphisms and gestational diabetes mellitus (GDM). Methods Ninety-nine pregnant women with GDM who were hospitalized or clinically examined in our hospital from June 2013 to June 2015 were enrolled in this study. 99 pregnant women with normal blood glucose during pregnancy were included in this study. The age, weight, body mass index BMI, body fat (BF) (%), gestational weight gain (kg), fasting blood glucose (mmol / L), 2 h postprandial blood glucose (mmol / L) μg / ml) and family history of type 2 diabetes mellitus (T2DM). The DNA samples were genotyped by polymerase chain reaction-restriction fragment length polymorphism (SNP): single nucleotide polymorphisms rs2576178 and rs10887800. Results In the age-matched case-control study, there was no significant difference in serum renal enzyme levels (P> 0.05). The rs10887800 gene polymorphism had a certain relationship with GDM. After many adjustments, the difference was still statistically significant (P <0.05). However, rs2576178 gene polymorphism was weakly correlated with GDM (P = 0.030), but no correlation was found after multiple adjustments (P = 0.09). Conclusion The rs10887800 polymorphism may be related to GDM. The polymorphism of rs2576178 is not correlated with GDM.