论文部分内容阅读
Objective:To studytherelationbetweenthecytoplasmicregionof eachsubunitof leukemiainhibitoryfactor(LIF)receptorandmitogen-activatedproteinkinase(MAPK)in humanleukemialineHL-60for studyingthe mechanism of leukemiacellproliferationdisorder.Methods:Wepreparedthechimericalreceptorwithexchangedcytoplasmicdo-main(190/130,130/190),expressediton themembraneof HL-60,andthenboundLIFwiththewildtypereceptorcompet-itively.Thecytoplasmicregionhomodimerization(190cyt-190cyt,130cyt-130cyt)initiatedintracellularsignaltransduc-tion,cellproliferationanddifferentiation.MAPKphosphorylationlevelwasobservedby meansof immunoblottingand immunobiochemistry.Results:Higherlevelof MAPKp42/p44phosphorylation(Thr202Tyr204)andfastercellproliferation weredeterminedingroup190/130comparedwiththewildtypereceptor.Thoseingroup130/190werelowerthantheoth-ers.Conclusion:Thecytoplasmicdomainof gp130involvesin theinductionof MAPKactivationandthecellprolifera-tionof HL-60.
Objective: To studytherelationbetweenthecytoplasmicregionof eachsubunitof leukemiainhibitoryfactor (LIF) receptorandmitogen-activatedproteinkinase (MAPK) in humanleukemialineHL-60for studyingthe mechanism of leukemiacellproliferationdisorder.Methods: Wepreparedthechimericalreceptorwithexchangedcytoplasmicdo-main (190 / 130,130 / 190), expressediton themembraneof HL-60, andthenboundLIFwiththewildtypereceptorcompet-itively.Thecytoplasmicregionhomodimerization (190cyt -190cyt, 130cyt-130cyt) initiatedintracellularsignaltransduc-tion, cellproliferationanddifferentiation.MAPKphosphorylationlevelwasobservedby meansof immunoblottingand immunobiochemistry.Results: Higherlevelof MAPKp42 / p44phosphorylation (Thr202Tyr204) andfastercellproliferation weredeterminedingroup190 / 130comparedwiththewildtypereceptor.Thoseingroup130 / 190werelowerthantheoth-ers.Conclusion: Thecytoplasmicdomainof gp130involvesin theinductionof MAPKactivationandthecellprolifera-tionof HL-60.