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为研制一种适应各种场合 ,尤其是在野外操作的基因枪 ,满足将目的基因导入动植物细胞 ,使之稳定表达并遗传 ,实现培育或改良新的优良品种。以压缩弹簧为动力 ,运用撞击发射原理 ,对裹着基因的微粒进行加速 ,使之获得足够的动能 ,去射击靶细胞。弹簧预压力不同 ,微弹的动能随之不同 ,可适应不同细胞的要求。利用所设计的装置 ,在常压下将GUS基因导入番茄叶片细胞 ,经检测 ,得到表达 ;将质粒psv- luc2 0转入小鼠的皮肤、腹部肌肉和肝脏内 ,也得到表达。实验表明 ,该构思是可行的 ,实验装置可以在常压下操作 ,能在各种条件下使用
In order to develop a gene gun to adapt to various occasions, especially in the field operation, to meet the target gene into animal and plant cells, so that the stable expression and genetic, to cultivate or improve new good varieties. To compress the spring as the driving force, the use of impact emission principle, the particles wrapped in genes to accelerate, so that sufficient kinetic energy to go to target cells. Different spring pre-pressure, micro-bomb kinetic energy followed by different, can adapt to the requirements of different cells. The GUS gene was introduced into tomato leaf cells under normal pressure by using the designed device and was detected to obtain the expression. The plasmid psv-luc20 was also transferred into the skin, abdominal muscles and liver of mice, and was also expressed. Experiments show that the concept is feasible, the experimental device can be operated under normal pressure and can be used in various conditions