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目的:研究乳腺癌MCF-7细胞系中SP细胞与非SP细胞表面标记的特点,并进行比较分析。方法:以体外培养的MCF-7细胞作为研究对象,采用Hoechst33342染色,并用流式细胞仪分选出SP细胞和非SP细胞,检测两种细胞的各种表面蛋白或抗原和细胞周期增殖状况。结果:乳腺癌MCF-7细胞株中SP细胞的含量为(10.1±0.84)%;分选后SP细胞的CD38、CD117和CD34表达率分别为(1.67±0.60)%、(0.43±0.22)%和(0.27±0.12)%,非SP细胞分别为(1.37±0.49)%、(0.42±0.24)%和(0.33±0.15)%,差异无统计学意义,P均>0.05;两者CD133、CD24、ABCG2的表达率分别为(34.33±7.03)%、(46.68±4.44)%、(85.82±2.48)%和(5.62±1.16)%、(75.38±5.14)%、(4.89±1.80)%,差异有统计学意义,P均<0.05;两者细胞中G0/G1、S、G2/M期细胞数分别为(81.34±6.69)%、(13.48±5.90)%、(5.18±2.42)%和(57.71±6.46)%、(23.14±6.46)%,(19.14±4.61)%,差异有统计学意义,P均<0.05;两者Ki-67表达率分别为(12.63±2.10)%、(34.37±4.71)%,差异有统计学意义,P=0.000。结论:乳腺癌MCF-7细胞系中含有比例较高的SP细胞,且SP与非SP细胞有可以分别鉴定和纯化的抗原差别。选择SP方法分离乳腺癌干细胞具有科学性。
OBJECTIVE: To study the characteristics of SP and non-SP cell surface markers in breast cancer MCF-7 cell lines and to compare and analyze them. Methods: MCF-7 cells were cultured in vitro and stained with Hoechst33342. SP cells and non-SP cells were sorted by flow cytometry. The surface proteins, antigen and cell cycle progression of the two cells were detected. Results: The percentage of SP cells in MCF-7 breast cancer cells was (10.1 ± 0.84)%. The percentage of CD38, CD117 and CD34 in SP cells was (1.67 ± 0.60)%, (0.43 ± 0.22)% (0.27 ± 0.12)% and (0.37 ± 0.49)%, (0.42 ± 0.24)% and (0.33 ± 0.15)%, respectively, with no significant difference , And the expression rates of ABCG2 were (34.33 ± 7.03)%, (46.68 ± 4.44)%, (85.82 ± 2.48)% and (5.62 ± 1.16)%, (75.38 ± 5.14)%, (4.89 ± 1.80)%, respectively (P <0.05). The number of G0 / G1, S, G2 / M phase cells in the two groups were (81.34 ± 6.69)%, (13.48 ± 5.90)%, (5.18 ± 2.42)% and 57.71 ± 6.46)%, (23.14 ± 6.46)% and (19.14 ± 4.61)%, respectively, with significant difference (all P <0.05). The Ki-67 expression rates were (12.63 ± 2.10)% and 4.71)%, the difference was statistically significant, P = 0.000. Conclusion: The breast cancer MCF-7 cell line contains a higher proportion of SP cells, and SP and non-SP cells have identified and purified antigen differences. It is scientific to choose SP method to isolate breast cancer stem cells.